Fabrication of micro/nanostructured Ti-6Al-4V surface and improved biocompatibility properties of hBMSCs via biomimetic peptide construction

Orthopedic implants tend to be personalized, lightweight, and functional, among which Titanium (Ti) alloy, especially Ti-6Al-4 V (TC4), has been widely used due to its high strength, excellent mechanical properties, resistant to corrosion and good biocompatibility. However, TC4 suffers from poor interaction between implants and bone tissues and performs deficient antibacterial properties. This work focuses on the design and fabrication of multi-level micro/nanostructured TC4 surface, so that to improve biological properties and promote easier interaction between implants and bone tissue. The adhesion, differentiation, and proliferation properties of hBMSCs have been systematically investigated through different surface configurations. It is proved that multi-level micro/nanostructured surfaces can form a uniform cell coverage layer and have good stimulation differentiation ability. In addition, Biomimetic functional coating has been constructed on the micro/nanostructured surface with peptide modification layer, regulating surface wettability, as well as affecting the adhesion and differentiation behavior of hBMSCs on TC4 materials. The antibacterial ability of the material can be improved and the influence mechanism of active reagents on surface wettability during peptide coupling process was analyzed. This work indicates a valuable prospect for further research and biomedical applications of micro/nanostructured TC4 surface.